BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2824

Peptides:

Sequences

Affinity Values

DCFVRCRVACA(2)
SCFALCRVPCQ(2)
KCFTACRVDCF(1)
SCFNRCRVPCF(1)
SCFDRCRVGGY(1)
QCFELCRVVCL(1)
RCFNLCRVGCL(1)
VCFPLCRVPCI(1)
ACFKACRVNCA(1)
ACFKHCRVACA(1)
DCFQGCRVFCS(1)
MCFDSCRVNCT(1)
TCFHRCRVTCI(1)
KCFQACRASCY(1)
RCFEKCRAFCY(1)
VCFSLCRAGCL(1)
VCFGVCRALCA(1)
VCFQLCRAQCI(1)
LCWTGCRVSCF(1)
GCWAPCRVGCY(1)
RCWTACRGLCF(1)
RCWQTCRVSCV(1)
QCWRSCRVNCL(1)
GCYTRCRVDCF(1)
VCYALCRVSCD(1)
DCYQLCRVSCE(1)
TCFRSCKVACY(1)
QCFQACKTLCW(1)

[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[7.5 ± 3.2]
[10.6 ± 0.5]
[NT]
[112.7 ± 22.1]
[NT]
[NT]
[NT]
[NT]
[NT]
[450 ± 75.5]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 23100545

Target Name:

Plasma kallikrein (EC:3.4.21.34)

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

XCX3CX3CX phage display library

Affinity Measurement Method:

Inhibition assay

Affinity Measurement Description:

The bicyclic peptides inhibited hPK with IC50s ranging from 7.5 to 450 nM. Bicyclic peptides were chemically synthesized and their inhibitory activity (IC50, nM) is indicated. NT denotes not tested.

Brief Description:

The library was joined and subjected to a first affinity selection with hPK. The second round of panning was performed using neutravidin-coated magnetic beads to prevent the enrichment of streptavidin-specific peptides.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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