BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2818

Peptides:

Sequences
CNTGSPYEC CTPSFSKIC

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (in vivo)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

3-4

Reference:

PMID: 25768046

Target Name:

The ligated left carotid artery in male C57/BL6J mice

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The ligated left carotid artery is the target for phage display, while the right artery serves as a normal flow control. Four days postligation, in vivo phage display was carried out. Phages were injected into the tail vein of the mice at a concentration of 2e11 plaque-forming units. After 2.5 h, mice were sacrificed by CO2 inhalation, and the carotid arteries were surgically isolated; phages were recovered from the vessels.

BiopanningDataSet Comments:

Titration of phage normalized to the protein content of the corresponding LCA or RCA was used to determine the relative uptake between the target and control arteries. All four consensus clones showed a significantly increased binding to the targeted LCA. ACTPSFSKIC showed average enrichment of 7.3-fold; SLSSYNGSALAS showed enrichment of 4.9; ACNTGSPYEC showed enrichment of 6.1, and GSPREYTSYMPH showed enrichment of 4.4 in the target artery.

Curator:

Curation Date:

0000-00-00

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