BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2815

Peptides:

Sequences
SNTRVAP(33.7%) RLGLAWG(14.5%) SNNFVAP(3.6%)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (in vivo)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 25762070

Target Name:

Bonelike matrix tissues of prostate cancer xenograft

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

CX7C fUSE5 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

For round 1, tumor-bearing mice received 2e9 transducing units (TU) of phage peptide library i.v. After 24 h of circulation, the mice were perfused with 10 mM PBS (pH 7.4), and tumors were collected. Soft tissue and bonelike matrix compartments of the tumor were separated physically, weighed, and homogenized for phage recovery. The recovered phage pool was amplified and subjected to another round of selection.

BiopanningDataSet Comments:

The two peptides, PKRGFQD and SNTRVAP, targeted the cell surface of androgen-independent prostate cancer cells in vitro, and homed to androgen receptor-null prostate cancer in vivo. Purification of tumor homogenates by affinity chromatography on these peptides and subsequent mass spectrometry revealed a receptor for the peptide PKRGFQD, α-2-macroglobulin, and for SNTRVAP, 78-kDa glucose-regulated protein (GRP78).

Curator:

Curation Date:

0000-00-00

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