BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2802

Peptides:

Sequences	Affinity Values
SWPLYSDASGLG(11) VSLHDDASGTHR(2) WLGESMISGWLY(1) TKFTDAGDTSFN(1) EHNDFPMYTWRP(1) WPDIEMDLGPLYA(1)	[0.72 ± 0.17] [0.92/0.85] [0.58] [0.75] [0.92] [0.85]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 17658545

Target Name:

Anti-Lpp20 monoclonal antibody L001

Template Name:

LPP20 lipoprotein

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

The binding activities of positive phage clones to the mAb L001 were measured by ELISA. The absorbance at 492 nm was measured and data shown were reproduced from the Fig. 5 in the reference. The phage library was served as the control, which had the value of 0.32 when binding to the mAb L001. In ELISA experiment, the peptide SWPLYSDASGLG appeared 11 times and the affinity value was presented as mean ± standard deviation, while VSLHDDASGTHR appeared 2 times and the two corresponding affinity values were separated by a slash.

Brief Description:

BiopanningDataSet Comments:

One mimotope (SWPLYSDASGLG) showed a good match with the Lpp20 proteins at 114–117aa (DASG) and the serum of mice induced by the phage clone clearly recognized Lpp20 protein. This mimotope provided an alternative approach for the diagnosis and development of a vaccine for H. pylori.

Curator:

Curation Date:

0000-00-00

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