BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2795

Peptides:

Sequences
LLSSKTL LSFPFPG FTSFSPY QATHFHS LASLPFR THVFSWI ACDPSPN TPSLHRS TAMARSA VALLPHH QSPPALL FSLLGSL VLLGPFP YPFSLLH SLGPQIK MSPTYLL DRAALSL ALTPQLL QTSPPLA FPLFGLS

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 25333662

Target Name:

Sera IgG from asymptomatic CVL and symptomatic CVL

Template Name:

Leishmania infantum

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The phage library first incubated with IgGs from healthy dogs. The supernatant containing the clones that were not adhered to the IgGs was recovered and transferred to a new tube, and this procedure was repeated for three times. Then, the supernatant incubated with IgGs purified from asymptomatic CVL. The bound phage clones were transferred to a new tube containing the IgGs purified from asymptomatic CVL, and the process was repeated for three times. After this, the recovered phage clones were transferred to a new tube containing the IgGs that had been purified from symptomatic CVL, and the process was also repeated for three times.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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