BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2791

Peptides:

Sequences	Affinity Values
NRPDSAQFWLHH(3) KVWLPPRHEHQY(1) KVFYPAAANPNQ(1) QALLEGNAKGGN(1) TADKLLYGLFKS(1) DEWDALLMRIRT(1)	[0.92 ± 0.06] [0.92 ± 0.03] [0.41 ± 0.06] [0.91 ± 0.04] [2 ± 0.05] [1.39 ± 0.12]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 25311190

Target Name:

Vaccinia virus strain NYCBOH

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Affinities of selected phages to vaccinia virus strain NYCBOH were measured by phage ELISA. The OD450-620 values were given and data shown were reproduced from the Fig. 1 in the reference. The wild-type M13KO7 phage (WT) was used as a control, which had the value of 0.3 ± 0.01 when binding to vaccinia virus strain NYCBOH.

Brief Description:

A negative selection was performed before every biopanning round. In the negative biopanning, the library was added to BSA-coated wells. Then the supernatant was transferred to target-coated wells. After the fourth round, binding phages were eluted and used for a further negative selection against HEp-2 cell lysate.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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