BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2787

Peptides:

Sequences

Affinity Values

RTEVPVLSFTSPLTG
GDVWLFKTSTSHFAR
AREYGTRFSLIGGYR
HAAFEPRGDVRHTLL
LGRAGQSYPSFARGL
PIFPVVSSSGSSSSP
PLSHGSVVYPRSSLG
RRDTVPRSLSAPLSW
PAVASTSSLIIDGPF
HPPLASVWHVSVPL
LHDFRSPIYASLLGF
AGDGGLGRVAAGARV
RVFHLWPHPTSTLSA
APLSYNFASMPFMSG
HPGWFDSAWFRAVSR
ARDSRCGGFLGCGVT
AMVRGFSFGMSRGSD
RSLWSDFYASASRGP
SYSVVNSPWCDGTCD
SRDGLHSFCYVGCPP
GVGDADGFIPVISAV
PVFFRLSPVTEGGGV
FPSYPFIAYSLQTPV
RRLPHLMPFEGSVFL
GPHFDYRTGLGWRFG
LGKGLTGSALSLSAL
YGVTPSPRSPWATAH
VFVDGARYSTASDSL
GAGIFGPWGVFAAVP
GYRSAFVPFVARGGH
RYRVGFTPGTIAAVL

[1.76]
[1.91]
[0.21]
[2.00]
[0.52]
[1.58]
[1.36]
[0.59]
[2.29]
[0.83]
[1.53]
[1.15]
[0.25]
[0.73]
[1.24]
[1.36]
[1.94]
[6.57]
[1.25]
[0.40]
[0.59]
[1.40]
[1.23]
[3.55]
[1.35]
[2.29]
[3.43]
[2.83]
[1.21]
[3.65]
[1.13]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning, in vivo)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 25250205

Target Name:

Human ovarian adenocarcinoma cell line SKOV-3

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

f5-15mer phage display library (X15)

Affinity Measurement Method:

Competition experiment,Micropanning assay

Affinity Measurement Description:

Micropanning assay was used to determine binding of selected phage clones to SKOV-3 and HS-832 cells. SKOV-3 or HS-832 cells were incubated with individual phage clones and eluted using 2.5% CHAPS. Phage binding was determined by titer and the SKOV-3 to HS-832 ratio was calculated and shown. In addition, competitive binding experiments indicated that the peptide RSLWSDFYASASRGP displayed a half maximal inhibitory concentration (IC50) value of 10.5 ± 1.1 μM.

Brief Description:

The library was pre-cleared from vasculature and non-tumor target binding phage according to previous methods. The pre-cleared phage library was screened against xenografted human OC SKOV-3 tumors in mice.

BiopanningDataSet Comments:

In the last round of selection, 31 individual phage clones were identified, and that phage clone RSLWSDFYASASRGP exhibited the highest SKOV-3-to-HS832 binding ratios of 6.57, indicating preferable binding to the human OC cell line.

Curator:

Curation Date:

0000-00-00

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