BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2755

Peptides:

Sequences
LLADTTHHRPWT(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (in vivo)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 20621144

Target Name:

Heart muscle and liver of mdx mouse

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In the first round, mdx mice were injected with 2e11 phage particles in PBS each via tail vein injection under anesthesia and sacrificed 2 h later. The heart was harvested. The phage particles homing to the heart muscle were harvested. For round 2 and 3 of the selection, 2e10 and 2e9 phage harvested from the heart muscle was injected into mdx mice respectively. For the last selection, liver tissue was also taken and phage within were amplified.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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