BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 273

Peptides:

Sequences
TVLTNPSTNHLS(2) AANMNMSRVSHT(2) TACHQHVRMVRP(1) STAELHFPMVFP(1) ASPPSYALPVTP(1) APHHTNITEIRI(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 16598852

Target Name:

Hepatocellular carcinoma cell lines BEL-7402

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

After the first round of panning, the phages were treated with normal liver cell line HL-7702 in the same way for reverse absorption, and the unbound phages were collected to eliminate the phages which can bind to liver cells. The residual phages were amplified and tittered for next round panning. After three rounds of panning and two rounds of reverse absorption the peptide sequences of randomly picked phage clones were analyzed by DNA sequencing.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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