BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2694

Peptides:

Sequences
FCAGALTCTTLP(8) TLNSNTTKPPLA(1) TATPDLTLYMPG(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 17236253

Target Name:

MH02 plasma

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Paramagnetic beads were precoated with a goat anti-human Fcλ-specific antibody and incubated (2 h at room tmeperature) with patient plasma (1:100). Beads were washed six times with PBS/0.25% gelatine/0.1% Tween-20 and rotated overnight at 4°C with 10 μL (1.5e11–2e11 particles) of the original phage peptide libraries. The next day, beads were washed extensively with PBS/0.25% gelatine/0.1% Tween-20 and the bound phages were eluted by pH shift [0.2 Mglycine-HCl (pH 2.2)/1 mg/mL BSA], neutralized with 1 M Tris-HCl (pH 9.1) and used for a negative selection with an HIV-negative plasma pool as described above. Supernatants of the negative selection were amplified and used for a second and third round of selection.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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