BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2652

Peptides:

Sequences
CKGGRAKDC CVMGSVTGC CRGEVLWSC

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning, in vivo)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 15133506

Target Name:

White fat

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In each biopanning round, an adult obese leptin-deficient [Lep(ob/ob)] female mouse was injected intravenously through the tail vein with 1.0e10 transducing units (TU) of the library. Phage (～300 TU/g in round 1 increased to ～e4 TU/g in round 3) were recovered after 5 min of circulation from subcutaneous fat and bulk-amplified for each subsequent round. Phage amplified after the third round of panning were enriched for fat-specific binders by adapting an in vivo subtraction step: a lean, C57BL/6 female was injected intravenously with 1.0e9 TU of phage selected in round 3. After 5 min, the unbound phage were recovered from circulation and amplified for the fourth and final round of biopanning in a Lep(ob/ob) female mouse.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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