BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2622

Peptides:

Sequences

Affinity Values

PRPGAPLAGSWPGTS(5)
DRWRPALPVVLFPLH(1)
ASSSYPLIHWRPWAR(1)
AAEWLDAFFVRHVDR(1)
GDVWLFLTSTSHFAR(1)
EGCSVSSVGALCTHV(1)
APCCSHLDASPFQRP(1)
PAQSNFVTWGYNVAV(1)
RASDVGSDVVPRYPF(1)

[24.8, 45.0]
[64.2, 36.1]
[53.5, 29.3]
[8.0, NT]
[5.2, NT]
[4.8, NT]
[3.6, NT]
[2.8, NT]
[1.5, NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (in vivo)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 11965539

Target Name:

Angiogenic vessel

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X15 fUSE5 phage display library

Affinity Measurement Method:

Binding assay

Affinity Measurement Description:

For affinity screening, B16BL6 cells were implanted subcutaneously into the posterior fank of 5-week-old C57BL/6 male mice. Each sample of phage clones (1.0e11 c.f.u.) was injected into tumor-bearing mice via a tail vein when the tumor size had become about 10 mm in diameter. Four minutes after injection, the phages that had accumulated in tumor tissue were recovered and titrated. Phage affinity was expressed as a fold of the control value obtained with random peptide expressing-phage. Both phage affinity against B16BL6 melanoma and phage affinity against Meth A sarcoma are shown. NT denotes not tested.

Brief Description:

The phage-displayed peptide library was injected into DAS model mice via a tail vein. Four minutes after injection, the phages that had accumulated in angiogenic vessels were recovered and titrated. Biopanning steps were repeated for five cycles.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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