BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2595

Peptides:

Sequences
SSLTSRDFGSWYASR

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 10426998

Target Name:

Estrogen receptor alpha, ERα

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Not determined.

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In the first round, immulon 4 96-well plates (Dynex Technologies, Chantilly, VA) were coated with streptavidin in NaHCO3 buffer (pH 8.5). Wells were blocked with bovine serum albumin (BSA) and then washed with TBST [10 mM tris-HCl (pH 8.0), 150 mM NaCl, and 0.05% Tween 20], and 2 pmol of biotinylated vitellogenin ERE was then added per well. Plates were washed with TBST, 3 pmol of baculovirus-purifed ERα was then added, and plates were incubated at room temperature for 1 hour. Estradiol or tamoxifen was then added (1 mM) along with phage library (containing about 1.5e9 phage) in TBST and incubated at room temperature for 1 hour. Affinity selection was repeated three times.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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