BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2580

Peptides:

Sequences
ECYINVPFTCMAGASAG(7) TECYKNVPEICAGASAG(1)

Sequences Features:

Motif:

Y-x-N

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 10400318

Target Name:

The SH2 domain of growth factor receptor-bound protein 2

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X12 phagemid-based fd phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Both MBP and GST fusions were used as affinity selection reagents in the experiments. To enhance the possibility of isolating SH2 domain-specific phage, the fusion partner-specific phage were "subtracyed" by co-incubating the phage with soluble GST or MBP during the affinity selection.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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