BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2539

Peptides:

Sequences	Affinity Values
ADAAPSPTPYLPRLS ATYRPVPAEFARKHL TITATDSPTPWPFER	[643] [425] [244]

Sequences Features:

Motif:

P-x-P-x(1-2)-P

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

3-4

Reference:

Target Name:

Anti-GAS CWPS monoclonal antibody HGAC 39

Template Name:

Cell-wall polysaccharide (CWPS) of group A Streptococcus (GAS), GAS CWPS

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X15 phage display library

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Absorbances are calculated as (A405 - A490) × 1000. The value of the wild-type phage vector f88-4 without a random peptide insert is 72.

Brief Description:

The library was affinity-selected on biotinylated mAbs that had been immobilized in avidin- or streptavidin-coated microwells.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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