BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2533

Peptides:

Sequences
FRSDVRFWHWSTPFM(11) VRVYFGFGPPPYFGG(9) WHWRHRIPLQLAAGR(2) RYWLYGDPASFPVNH(2)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 9877157

Target Name:

Anti-GD1α monoclonal antibody KA17

Template Name:

Ganglioside GD1α SIAT7-E and SIAT7-F

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X15 fUSE5 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Each round of biopanning was performed on the 96-well microtiter well (Falcon 3072) coated with streptavidin. The biotinylated KA17 was adsorbed onto the streptavidin-coated well, and affinity-isolation of phages from the peptide epitope library by four cycles of biopanning was carried out.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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