BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2522

Peptides:

Sequences
SKLLYNYGACRTGCYMAGR ELISSCLVWSARGCLFGGGI VMDECVFSSISVLFCNHMLH

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 16517852

Target Name:

Anti-EBV monoclonal antibody A3

Template Name:

Epstein-Barr virus, EBV

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X20 fUSE5 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Coated wells were blocked with 5% BLOTTO (milk powder diluted in phosphate-buffered saline [PBS]), and the 20-mer phage peptide library was preincubated in 1% BLOTTO for 15 min to remove any milk-binding phage before it was added to the MAb-coated wells.

BiopanningDataSet Comments:

To assess their diagnostic value, a panel of 62 individual EBV IgM sera for their reactivities was screened with the peptides alone. For all peptides, there was a clear distinction between the EBV-positive and the EBV-negative samples, resulting in 100% specificity. The sensitivities were 88%, 85%, 71%, and 54% for peptides F1 (YTDSSMAVTLMKFASNFLF), A3 (SKLLYNYGACRTGCYMAGR, ELISSCLVWSARGCLFGGGI and VMDECVFSSISVLFCNHMLH), gp125 (GGWYSFDSPYLMSITEMRLR), and A2 (DNYWSFSDSTYWTLRYSSG), respectively. Any combination of peptides increased the sensitivity, indicating that individual peptides react with different subsets of antibodies. Furthermore, when the F1 and the gp125 peptides were coupled to bovine serum albumin and screened against 216 serum samples, there were dramatic improvements in sensitivities (95% and 92%, respectively) and little cross-reactivity with the other peptides encountered during acute viral infections, including rheumatoid factor. This study shows the potential for the use of peptide mimotopes as alternatives to the complex antigens used in current serodiagnostics for EBV infection.

Curator:

Curation Date:

0000-00-00

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