BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2490

Peptides:

Sequences

ADITDPMGA(2)
AVSPNVHDG(2)
AAPDLQDAM(1)
ADRLNSDAG(1)
ADRPSTTSL(1)
ADPPRTVST(1)
ADRPSMSPT(1)
ADRTSNAST(1)
ADKSYIPSS(1)
AVRNPSHHS(1)
ADPTPRGHS(1)
ADPTRQPHS(1)
AEHQNSAGP(1)
ADARSAGAIS(1)
ADSKNAGPM(1)
AETKFSGSA(1)
ADPKGSGVT(1)
AGLTSPNDM(1)
AVGTHTPDS(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 12089009

Target Name:

Phytophthora capsici zoospore

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

f8-8mer phage display library (X8)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In the affinity selection procedure, P. capsici zoospores were added to phage in the presence of 50 mM LiCl, which prevents zoospore encystment.

BiopanningDataSet Comments:

The abilities of phage clones to induce encystment varied. For example, at a concentration of 2.5e9 virions/μl, clones Pc78 (displaying ADPPRTVST), Pc87 (displaying ADRPSMSPT), Pc64 (displaying ADITDPMGA), and Pc42 (displaying AVSPNVHDG) induced >80% encystment within 20 min. Conversely, only about 20% of zoospores exposed to Pc56 (displaying AAPDLQDAM), Pc45 (displaying AEHQNSAGP), Pc15B (displaying AGLTSPNDM), or the fuse5 phage vector were encysted during this period. In solution, 200 μM peptide 87, corresponding to phage Pc87, induced complete encystment of zoospores. In contrast, peptide 56, corresponding to the ineffectual phage Pc56, did not induce significant encystment at the same concentration. Peptide 42, corresponding to phage Pc42, induced an intermediate level of encystment.

Curator:

Curation Date:

0000-00-00

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