BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2488

Peptides:

Sequences

SVSVGMKPSPRP(3)
ASPMPTAKLRFA(1)
NWTTNRPNNTSP(1)
MNNDHVPSVAQG(1)
AYWSNPESHPXQ(1)
SNPFSKPYGLTV(1)
WDSNTYTPRPLM(1)
ATNPTHPGHLPV(1)
NPSLHKPLTISI(1)
NPYQHRNWAYVG(1)
HARENSLPWLHA(1)
YPHYSLPGSSTL(1)
LPLALPRHNASV(1)
SSLKLHQTPLDA(1)
SSLEPWHRTTSR(1)
GSIALSSWLSLP(1)
GNFQSESYFVPH(1)
GLHESTFTQRRL(1)
FSHELSWKPRKA(1)
NYLHNHPYGTVG(1)
LNYFTLSSKRE(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (in vivo)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 19276080

Target Name:

SAS-derived tumors

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

SAS cells were injected subcutaneously into the dorsolateral flank of 4–6-week-old SCID mice to produce oral cancer xenografts. The phage-displayed peptide library was injected intravenously into the tail vein of SCID mice bearing size-matched SAS-derived tumors.

BiopanningDataSet Comments:

From 30 random selected phage clones, 23 phage-displayed peptide sequences were identified. Six novel peptides (NYLHNHPYGTVG, SNPFSKPYGLTV, GLHESTFTQRRL, YPHYSLPGSSTL, SSLEPWHRTTSR and LPLALPRHNASV) were identified as being able to recognize tumor vasculature but not normal blood vessels in severe combined immunodeficiency (SCID) mice bearing human tumors. These tumor-homing peptides also bound to blood vessels in surgical specimens of various human cancers. The peptidelinked liposomes containing fluorescent substance were capable of translocating across the plasma membrane through endocytosis. With the conjugation of peptides and liposomal doxorubicin, the targeted drug delivery systems enhanced the therapeutic efficacy of the chemotherapeutic agent against human cancer xenografts by decreasing tumor angiogenesis and increasing cancer cell apoptosis. Furthermore, the peptide-mediated targeting liposomes improved the pharmacokinetics and pharmacodynamics of the drug they delivered compared with nontargeting liposomes or free drugs. It indicates that the tumor-homing peptides can be used specifically target tumor vasculature and have the potential to improve the systemic treatment of patients with solid tumors.

Curator:

Curation Date:

0000-00-00

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