BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2472

Peptides:

Sequences

Affinity Values

SSQTDWRKIFQSL
SSSTDWLNVWRQL
SSATDWGRVYSIL
SSASYAPWPIYFA
SSGAFKPWPVYSF
SRQVEVFKPWPVY
SSSFKPWPIYLGS
SSEPFSVWPIYKH
SSSVPFAPWPVYA
SSTSLPFNRWPIY

[0.36]
[0.15]
[0.94]
[0.76]
[0.36]
[0.44]
[0.52]
[0.44]
[0.62]
[0.22]

Sequences Features:

Motif:

T-D-W-x(2)-[IV]-[FWY]-x-L, [FY]-K-P-W-P-[IV]-Y

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

3/4

Reference:

PMID: 10662687

Target Name:

β-glucosidase

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Twelve different M13 phage libraries pool

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Absorbance at 405 nm was measured.

Brief Description:

Biotinylated-target protein and phage were incubated together in solution prior to capturing the target-phage complex on streptavidin-coated paramagnetic particles. Or the target protein was biotinylated and captured on streptavidin-coated 96-well plates.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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