BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2467

Peptides:

Sequences

Affinity Values

FDRLSLADVEQVSIEEAKAWALVQLRDW(11)
WRTDVRWLSEFEEAEVEGKRPGVWLYGT(9)
SSRADDVVWVSTGEEAAWRRQWLDLRTV(9)
ADAVLPWGSSARWDIGGWQVASGGGTQA(5)
GGGSPVRAEWAQDIRSRSEREMLVWLGK(2)
AEPGVRERTEMEMGEIFGNLDLCMARRW(1)

[2.742, 3.362]
[6.704, 7.684]
[2.339, 4.168]
[24.903, 26.285]
[20.854, 23.347]
[13.420, 13.735]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 9762658

Target Name:

IgG from cerebro spinal fluid of multiple sclerosis (MS) patient 1

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X28 and X28 M13 phage display library pool

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Binding of the selected phagotopes to the CSF and serum IgG from patient 1 was detected by ELISA. For each sample equal amounts of IgG were used and antibody recognition of the tested phagotope and wild type phage was measured. Average values (A405 nm) from two independent experiments have been determined. The background signal obtained on the wild type phage was subtracted. Results are expressed as the ratio between the subtracted value of the tested phagotope obtained by using CSF IgG and that observed using serum IgG (CSF A 405 nm/serum A 405 nm). These data shown were reproduced from the graph. Data obtained by using samples from the same patient at 2 years time interval are also shown.

Brief Description:

For each selection, CSF IgGs were immobilized using magnetic beads coated with goat anti-human IgG Fc-specific Ab.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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