BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2439

Peptides:

Sequences	Affinity Values
HAIYPRH AKPGYLS ALTPTPP ANYPREP ATTVPAS LTTHYKL NLVNLLP KCCFPAQ	[1.10 ± 0.08] [>1.9] [>1.9] [>1.9] [>1.9] [>1.9] [>1.9] [NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (in vivo)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 22463021

Target Name:

Colonic adenomas

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Flow Cytometry

Affinity Measurement Description:

Binding to isolated colonic epithelial cells from adenomas for each phage clone was analyzed using flow cytometry. A common 7-mer phage that expresses the sequence HAIYPRH is a known contaminant and was run to ensure the validity of the assay. The T/B ratio for clone HAIYPRH was found to be 1.10 ± 0.08, indicating minimal binding from a nonspecific clone, as expected. Six phage clones with a T/B ratio (target to wild-type phage) >1.9 were identified as ALTPTPP, NLVNLLP, ANYPREP, ATTVPAS, LTTHYKL, and AKPGYLS. ND denotes not determined.

Brief Description:

A seven-month old CPC, Apc mouse, was injected via tail vein with the phage display library. A total of three rounds of phage biopanning were performed, with amplification of the recovered eluate after each biopanning round.

BiopanningDataSet Comments:

After three rounds of in vivo phage biopanning, 42 individual phage clones were sequenced from the third round of biopanning and were individually amplified and conjugated to 5'-FITC.

Curator:

Curation Date:

0000-00-00

First |

Prev |

2439

| Next

| Last