BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2410

Peptides:

Sequences
TEPSTRGSWKFW(13) SVPQESTIRATK(5) SGHTHYYPAETN(4) LCNTYLPLARST(3) VPILAHPSTGHS(2)

Sequences Features:

Motif:

SENRKVPFYSHS

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 24468276

Target Name:

The Domain III of the Japan Encephalitis Virus Envelope Protein, E DIII

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

The homologous alignment of peptides displayed by frequently encountered phages was analyzed, resulting in the identification of a highly conserved 12 amino acid peptide sequence that was also synthesized and named P3 (SENRKVPFYSHS). The inhibitory ability of the peptides against JEV infection were determined by plaque assays, qRT-PCR and WB. The plaque assay results showed that P3 significantly inhibited JEV infection in a concentration-dependent manner, whereas P1 (TEPSTRGSWKFW) inhibited JEV infection only at high concentrations. P2 (SVPQESTIRATK) did not show any inhibitory effect. Besides, P3 bound to E DIII with a Kd of 6.06 × 10 (-6) M, indicating that P3 has a higher affinity for E DIII. Also P3 interfered with JEV binding to the cell receptor. Eventually A model of the E DIII/P3 complex was developed by molecular modeling based on the available E DIII structure.

Curator:

Curation Date:

0000-00-00

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