BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2397

Peptides:

Sequences
EVDGRWWIVETFLAKWDHMA

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 11075354

Target Name:

Insulin-like growth factor-binding protein 1, IGFBP-1

Template Name:

Insulin-like growth factor I, IGF-I

Structure of Target-Template Complex:

2DSQ

Structure of Target-Peptide Complex:

Not determined.

Library Name:

X20 M13 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

For the first three rounds of selection, IGFbp-1 was covalently linked to biotin using EZ-Link NHS-SS-biotin (Pierce). The biotin-SS-IGFbp-1 was first allowed to bind to immunosorbent plates coated with NeutrAvidin (Pierce). The fourth round of binding selection was carried out on immunosorbent plates directly coated with IGFbp-1.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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