BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2391

Peptides:

Sequences
CGTFAHPQC

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 18533899

Target Name:

Streptavidin

Template Name:

Biotin

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The phage library was incubated with streptavidin. Elution by sonication was introduced. After 25 washings with PBST and 4 with glycine-HCl (0.2 M, pH 2.2), another 100 μl of glycine-HCl were added to the microtiter plate well, and the covered plate was immersed in a sonicator water bath (50 kHz) for 10 min. Three rounds of selection were performed.

BiopanningDataSet Comments:

A lot of phage clones were recovered. Phage-displayed peptide sequences were determined if the ELISA signal of individual clones exceeded the blank by at least fourfold. So phage clones displayed peptide CGTFAHPQC were obtained.

Curator:

Curation Date:

0000-00-00

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