BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2378

Peptides:

Sequences

GHTHKAM(1)
GTSQFQL(1)
HPPLLAT(1)
HSMPTLH(1)
HSSNAQV(1)
HVPTVLS(1)
IQQLKPP(1)
ITPAALS(1)
LNLDRKP(1)
LPNPRNL(1)
LQKTFMV(1)
LSAKITL(1)
LSTATVR(1)
LTNSEWD(1)
LTQQPPR(1)
LWATRHI(1)
LYALVPS(1)
NQLLGST(1)
QKPVYTR(1)
QSFGVQL(1)
SFPATKT(1)
SHDVTPQ(1)
STPLIPT(1)
TPALFPY(1)
TSPPRYL(1)
VIHVKSP(1)
VSHTQAN(1)
YPRTISA(1)
YSNAPTH(1)
YSPHPYP(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning, competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 24365751

Target Name:

IgE of the peanut allergic patient no. 2208

Template Name:

Allergen Ara h 1, clone P41B

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In negative selection, the library was incubated with rabbit-α-human IgE. For positive selection, IgG4 of the peanut allergic patient no. 2208 were used and phages were eluted by competitive immunoscreening, by adding phages were eluted by competitive immunoscreening by adding allergen (intact and digested Ara h 1) per 1 mL of beads. Three rounds of positive selection were performed.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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