BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2375

Peptides:

Sequences

AQSQFNS(3)
AKTSSNV(1)
APLPKLM(1)
DQWHRAP(1)
FGFPTTS(1)
FPTPRVA(1)
HQPQQLF(1)
ISVNIQA(1)
IVQHTVP(1)
KPWLPQH(1)
NAHHTYP(1)
NHTTLKA(1)
NPLPSQL(1)
QPVPPRL(1)
SDRFPPK(1)
SDYHRVM(1)
SLDTCLR(1)
SPSPQRS(1)
TGGVWSK(1)
YNPAVIA(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning, competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 22555070

Target Name:

IgE of animal nr. 53

Template Name:

Allergen Ara h 1, clone P41B

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In negative selection, the library was incubated with mouse-α-rat IgE. For positive selection, IgE of animal nr. 53, rat immunized with intact Ara h 1, were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (digested Ara h 1). Three rounds of positive selection were performed.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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