BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2372

Peptides:

Sequences
TASGLYS(4) GPPPLPK(1) HHMHTTR(1) IAMKPLA(1) LPSLPRI(1) LQSKTLH(1) NTSNPYT(1) QATFSHS(1) STFLPHP(1) VPPQLSR(1) VTAPFRV(1) VYPMAMS(1) YHGSVSL(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning, competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 22555070

Target Name:

IgE of animal nr. 49

Template Name:

Allergen Ara h 1, clone P41B

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In negative selection, the library was incubated with mouse-α-rat IgE. For positive selection, IgE of animal nr. 49, rat immunized with intact Ara h 1, were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (digested Ara h 1). Three rounds of positive selection were performed.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

First |

Prev |

2372

| Next

| Last