BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2367

Peptides:

Sequences
FSPIVLY(2) SPITEFY(2) KSIYHQW(1) LPSRGLF(1) LTDLTQK(1) QSLTRLP(1) SPILAHY(1) TFKLHPI(1) TPPRVHL(1) VLPGSKS(1) WESRGVF(1) YPPKPLH(1)

Sequences Features:

Motif:

S-P-I-x(2)-[HY]-Y

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning, competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 22555070

Target Name:

IgE of the peanut allergic patient no. 2305

Template Name:

Allergen Ara h 1, clone P41B

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In negative selection, the library was incubated with rabbit-α-human IgE. For positive selection, IgE of the peanut allergic patient no. 2305 were used and phages were eluted by competitive immunoscreening, by adding 25 μg allergen (intact Ara h 1). Three rounds of positive selection were performed.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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