BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2351

Peptides:

Sequences
LGDSSNSQVSLN(1) LPHSAVMAQLTY(1) STSHHHHPSAPS(1) TLHAHQHHQPST(1) WMNGPVSIRTWS(1) WPYNHHHRTPSP(1) YSHHHMHTPHTR(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 24287902

Target Name:

Quinoprotein glucose dehydrogenase

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The phage display peptide library was incubated with Ni-agarose magnetic beads in microtubes to eliminate phages binding to the beads or the tubes. In the first round, 120 μg of GB-His in immobilization buffer was added to 120 μ L of Ni-agarose magnetic.The library was subsequently added to 120 μg of GB-His immobilized Ni-agarose magnetic beads. In the second round, the first-round-eluted phages were incubated with 48 μg of GB-His. In the third round (negative-selection round), the phages were incubated with DEE-His.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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