BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2348

Peptides:

Sequences
CTYYWPLPC CSWYWPLPC CLMTSQFRC CWPIKVGWC

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 24265799

Target Name:

Ephrin type-A receptor 4

Template Name:

Type A/B ephrins

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

After four rounds of selection on EphA4, we detected an approximately 75-fold enrichment of phage binding to EphA4. In total, 48 of the individual clones that were tested for the binding to EphA4 Fc, and 6 of them showed significant binding when compared with the BSA negative controls. All 6 of these EphA4-binding clones displayed four different cyclic peptide sequences. The cyclic nonapeptide, TYY, CTYYWPLPC, binds to EphA4, but not to EphB4, and selectively inhibits the binding of ligand ephrinA5 to EphA4. The TYY cyclic peptide was evaluated for its cytotoxic effects on HUVECs, and it effectively inhibits HUVEC tubule formation activity.

Curator:

Curation Date:

0000-00-00

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