BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2319

Peptides:

Sequences

SQMHLRY(1/25)
KGFHGYT(1/25)
LLTKNSL(1/25)
KPRSVSG(11/25)
VMAPHKY(1/25)
QRHTRPN(1/25)
YLPPYKS(1/25)
DHPAVAQ(1/25)
SSPDKSF(1/25)
HRPYIAP(1/25)
ASEQVHM(1/25)
SRLTMHT(1/25)
HEVSDYL(1/25)
THSKPLH(1/25)
NKITQHS(1/25)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 23784990

Target Name:

Ceramic fluorapatite

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The original phage library was diluted in 1 ml TBST (0.1%) buffer and incubated with the blocked empty column (without CFA sample) at room temperature. After 1 h of incubation, the phage solution was discarded. The discarded solution contains the phages that did not show strong affinity to the column materials and was used for the selection of CFA-binding phages.

BiopanningDataSet Comments:

The peptide F5-4 ((KPRSVSG)), displayed by more than 40% of the phages analyzed in the fifth round of selection, was subjected to further studies. Selectivity of the peptide for the chemical composition and morphology of CFA was assured by the adsorption studies. The dissociation constant, obtained from the F5-4/CFA adsorption isotherm, was in the micromolar range, and the maximum capacity was 39.4 nmol/mg.

Curator:

Curation Date:

0000-00-00

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