BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2295

Peptides:

Sequences
YEQDPWGVKWWY(8) HLSWLPDVVYAW(2)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 24046373

Target Name:

M2 macrophages

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 and Ph.D.-C7C phage display library pool

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

For the subtractive pans, 2.0e11 pfu of the amplified eluate was first applied to receptor-cleared M1 cells. M1 cells were centrifuged, and the supernatant was applied to M2 cells. Phage was eluted from M2 cells and amplified. Subtractive panning was repeated three times, using the amplified eluate from the previous pan as the starting library for each round.

BiopanningDataSet Comments:

M2pep (YEQDPWGVKWWY) shows selective binding and internalization in M2 macrophages but with minimal M1 macrophage interaction. We demonstrate this peptide's ability to identify murine M2-like TAMs within mixed cell populations isolated from mouse colon carcinoma tumors and to accumulate in TAMs after i.v. injection into tumor-bearing mice. We show that i.v. administration of M2pep fusion peptides with KLAKLAKKLAKLAK (KLA), a proapoptotic peptide, to tumor-bearing mice selectively reduces TAM populations and prolongs survival.

Curator:

Curation Date:

0000-00-00

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