BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2291

Peptides:

Sequences

RRWVRYPVHLHSPIV(1)
PPYHRFWRGHRHAVQ(12)
HRISHFAHRYLARLH(2)
WHWRHRIPLQLAAGR(2)
GWHSLLHSRYHRIAA(1)
FVWVRFHRLPRQIYT(1)
WHKYFLRRPLSVRTR(4)
VPMALNHGVYVMVSS(1)
RKWFLQHRRMPVSVL(6)
SGRRHLHRHHIFSLP(2)
GWITFHRRHHDRVLS(1)
RLHGHRSHRFTHVAQ(1)
KRIWFIPRSSWYERA(3)
MPLSRYWWLFSHRPR(1)
RHLSHFKWLRSHGLD(1)
RRFHFHSRMVAVDNS(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 17804251

Target Name:

Transforming growth factor beta-1, TGF-beta-1

Template Name:

Transforming growth factor beta receptor type 3, TGF-beta receptor type 3, TGFR-3

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

f5-15mer phage display library (X15)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Each selection was performed on the streptavidin-coated plates.

BiopanningDataSet Comments:

In vitro and in vivo experiments were performde to valuate their capacity to neutralize TGF???21. A murine model of acute liver damage was used to test the in vivo activity of all 20 phage-derived peptides. As found in vitro, P17 (KRIWFIPRSSWYERA) was also the most active in vivo followed by P6 (PPYHRFWRGHRHAVQ), P4 (RLAHSHRHRSHVALT), P14 (SGRRHLHRHHIFSLP) and P11 (WHKYFLRRPLSVRTR). Other peptides such as P1 (DRRIFWWSLRSAPGA), P3 (RFFTRFPWHYHASRL), P7 (HRISHFAHRYLARLH), P9 (GWHSLLHSRYHRIAA), P15 (GWITFHRRHHDRVLS), P19 (RHLSHFKWLRSHGLD) and P13 (RKWFLQHRRMPVSVL) were without effect on the expression of collagen type I mRNA whereas peptides, P2 (HVRLHHYLRHRSLPN), P5 (RRWVRYPVHLHSPIV), P8 (WHWRHRIPLQLAAGR), P10 (FVWVRFHRLPRQIYT), P12 (VPMALNHGVYVMVSS), P16 (RLHGHRSHRFTHVAQ) and P18 (MPLSRYWWLFSHRPR) enhanced expression of collagen type I mRNA.

Curator:

Curation Date:

0000-00-00

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