BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2279

Peptides:

Sequences
RGRGAG LGRRRS IRGRWA AGRSAF GRTDFW IKRNIT PRGRGG NKYARQ YHIKRS

Sequences Features:

Motif:

[GA]-R-x-[AG]

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 7644467

Target Name:

Tissue-type plasminogen activator

Template Name:

Plasminogen, EC=3.4.21.7

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

f3-6mer phage display library (X6)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

For positive selection, the phage library was digested overnight at 37°C by concentrations of t-PA varying from 10 to 100 μg/ml. The reaction mixture was then placed on ice, bovine serum albumin was added to 0.1%, and 100 μg of mAb 179 and 10 μg of mAb 3-E7 were added. After 30 min on ice, 100 μl of Pansorbin cells was added, and the reaction mixture was rotated at 4°C for 1 hr. The mixture was microcentrifuged for 2 min, and the supernatant was recovered to repeat the Pansorbin adsorption.

BiopanningDataSet Comments:

Curator:

Curation Date:

0000-00-00

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