BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2271

Peptides:

Sequences
QCTNFISDHECH(4/7) SCDGFYTGPACM(2/7) QCVETWNRIECK(1/7)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 10975844

Target Name:

Monoclonal antibody TP25.99

Template Name:

β2m-free or β2m-associated HLA class I heavy chains

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

LX-8 phage display library (XCX8CX)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Microwells were coated with avidin. The first round of panning was performed using 1.0e12 phage particles in TBS 50 and 1mg biotinylated mAb TP25.99 per well. The subsequent three rounds of selection were conducted using 1.0e10 phage particles and 0.1μg biotinylated mAb per well.

BiopanningDataSet Comments:

We have mapped the conformational and the linear determinants recognized by mAb TP25.99 on HLA class I heavy chains. This information contributes to our understanding of the structural relatedness of distinct antigenic determinants defined by a mAb and of the conformational changes in HLA class I heavy chains induced by their association with β2m.

Curator:

Curation Date:

0000-00-00

First |

Prev |

2271

| Next

| Last