BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2262

Peptides:

Sequences
CKTVPDNHC CDSPLTRNC CIGKFLYRC

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 23502767

Target Name:

MD-2 mimic peptide, NH2-FSKGKYKCV-COOH

Template Name:

Lipopolysaccharide, LPS

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Western blot

Affinity Measurement Description:

The Western blot (WB) analysis showed clearly that AP6 (CKTVPDNHC) and AP11 (CDSPLTRNC) indeed bound the MD-2 molecule.

Brief Description:

BiopanningDataSet Comments:

After 3 rounds of selection, 15 plaques were identified and their culture supernatants'ability to inhibit the production of cytokines was tested in PMA-induced U937 cells stimulated with LPS. Of the 15 clones, #5, #6, #8, #10, and #11 demonstrated good inhibition of TNFa and IL-6 production. We selected clones CKTVPDNHC, CDSPLTRNC, and CIGKFLYRC for further investigation as these 3 clones shows more inhibition effects than others.

Curator:

Curation Date:

0000-00-00

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