BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2256

Peptides:

Sequences

CKSLEYSYC(18)
CKSPENSYC(6)
CTNTLSNNC(5)
CTNTLNYNC(2)
CKSTENSYC(1)
CKSPENYYC(1)
CKSPENYNC(1)
CKSLENYYC(1)
CKSMENSYC(1)
CKYTESYNC(1)
CKYLENSYC(1)
CKSPENSSC(1)
CNNTQSYNC(1)
CTTPGNYNC(1)
CKTRENSYC(1)
CKSTQNSNC(1)
CKNTENYNC(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 20709321

Target Name:

Pb2+

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-C7C phage display library (CX7C)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

The phage library was first screened against uncharged monolithic column. Three rounds of main biopanning were conducted against Pb2+ as a target. The phage sample from the third round of positive selection was screened against uncharged monolithic column (post-negative screening I). After three rounds, the amplified phages were used in the next post-negative screening (post-negative screening II) against various other metal ions. Three rounds of negative selection against Cu2+, three rounds against Ni2+, two rounds against Co2+ and one round against Fe3+ were conducted.

BiopanningDataSet Comments:

Sixty individual clones were sent for sequencing and 44 DNA sequences were obtained.

Curator:

Curation Date:

0000-00-00

First |

Prev |

2256

| Next

| Last