BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2250

Peptides:

Sequences

Affinity Values

ETAPLSTMLSPY(17)
SVSVGMKPSPRP(14)
TLHPSVLSYVLK(13)
EVFWPLNAPRLL(11)
ASTNVFARPMYL(8)
APKYSLSDLYLN(7)
TPPPRDASLSRW(7)
SWQITYPISPRS(5)
TALPNHWSDASP(4)
SSFPQVIPLDYL(4)
TDSYHVASARQP(4)
YTFMPELTTPRT(4)
NSFNYAPLLMPR(3)
SGFFEPQHSHPL(3)
TTFSPPSPLSSI(3)
KTAPTPYALVHD(2)
FNNHYPAAATYP(1)
SWQIPYPISPRS(1)
YTTWPFTSLQLD(1)
ETAPPTPYSVMF(1)
TTFNPLYLRLDT(1)
TSPYHLLHAHLQ(1)
SSFVALSISPSM(1)
SPQTDGLVSTPS(1)
SNFMHNTRIWSH(1)
STYSHPLSLRPD(1)
AWTWVLPSSIRA(1)
AFMETTSQNAWL(1)
QIEKISQHLDMH(1)
TWNQPYIPPLYP(1)
YASPPNPSLRLT(1)
YHGLTPVRYVSV(1)
ANLSSHSSPGDS(1)
TTLQFTGQTNKT(1)
HNIGTWGPKSHL(1)
QIEESFVRGHTT(1)
YTFDPQIRPAGL(1)
YERSILPFSHVF(1)
YPSVTFPTQTLL(1)
SPWYMTPSPNTA(1)
HISVINYTTKIS(1)
MNVTVSGRLSGP(1)
TIPYPFSLLNNP(1)
PFLYSQVAWRS(1)
YQEETPASSFSR(1)
NSSQLAPYTTHR(1)
HNGLPNFFQTRL(1)
EAAPNFYPPLTF(1)
DLFQFAFPLNTI(1)
FTFSYAESVSYF(1)

[1.924 ± 0.074]
[0.772 ± 0.028]
[2.025 ± 0.194]
[2.293 ± 0.179]
[0.571 ± 0.063]
[0.884 ± 0.153]
[1.001 ± 0.096]
[1.628 ± 0.084]
[1.874 ± 0.123]
[2.293 ± 0.196]
[1.633 ± 0.327]
[1.554 ± 0.074]
[1.633 ± 0.090]
[1.298 ± 0.055]
[1.237 ± 0.265]
[1.385 ± 0.127]
[0.720 ± 0.164]
[0.910 ± 0.122]
[2.204 ± 0.401]
[1.940 ± 0.095]
[2.473 ± 0.090]
[1.792 ± 0.084]
[2.393 ± 0.429]
[1.132 ± 0.166]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]
[NT]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 23792224

Target Name:

MDR GC cells SGC7901/ADR or SGC7901/VCR

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

In phage ELISA, unrelated phages (URPs, the amplified phages from original phage peptide library) and PBS were used as negative controls. Absorbance value was determined. Data were reproduced from the graph and expressed as mean ± SEM (n=3). The absorbances of URPs and PBS were 0.239 ± 0.028 and 0.093 ± 0.043, respectively. NT denotes not tested.

Brief Description:

Whole-cell subtractive screening from a phage display 12 peptide library was performed on MDR GC cells with a drug sensitive GC cell line, SGC7901, and immortalized gastric mucosal cells (GES) used as control.

BiopanningDataSet Comments:

After four rounds of panning, 200 phage colonies were picked randomly and identified for MDR reversal capability using the MTT assay. GMBP1 (ETAPLSTMLSPY) could inhibit the tumorigenesis of gastric cancer MDR cells and might have the potential to re-sensitized gastric cancer to chemical drugsin vivo.

Curator:

Curation Date:

0000-00-00

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