BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2204

Peptides:

Sequences	Affinity Values
THSPVHVLAEHI(5) YHSDVHCANTCN(5) SHSVVHVTKNQY(3) WHTGVHISAKPG(2) ELTNAQIKLLWE(1)	[3.273 ± 0.225] [3.351 ± 0.120] [2.410 ± 0.172] [3.783 ± 0.120] [2.090 ± 0.172]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 12413654

Target Name:

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

Phage ELISA was utilized to measure the ability of the phage-borne 12-mer peptides to bind Par17. ELISA 96-well plate was coated with Par17 (10–20 μg/ml) and ten wells were coated. Absorbance at 405 nm was measured. Mean values and standard deviations were taken for the ten coated wells and shown. Data were reproduced from the bar graph.

Brief Description:

BiopanningDataSet Comments:

The peptide consensus sequence XHSXVHΦ was enriched , where X can be any amino acid, and Φ is any hydrophobic residue.

Curator:

Curation Date:

0000-00-00

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