BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 2013

Peptides:

Sequences	Affinity Values
HSACDLPMHPMC(6) HSACDLLMHPMC(1) HSACDLPKAPWC(1)	[0.688 ± 0.033] [0.659 ± 0.033] [0.702 ± 0.035]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 22945508

Target Name:

CD59

Template Name:

T-cell surface antigen CD2

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

In phage ELISA, the absorbance at 490 nm was determined. Data were reproduced from the graph and expressed as means ± SDs. The absorbances of phages with the same sequence were calculated as mean ± SD. The absorbance of the wild-type M13 phage was 0.121 ± 0.006.

Brief Description:

BiopanningDataSet Comments:

The affinities of the 13 phage clones to human CD59 were measured by ELISA.Eight clones showed a high binding to CD59.All sequences exhibited 9 hydrophobic residues.

Curator:

Curation Date:

0000-00-00

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