BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1989

Peptides:

Sequences

NSWTNASLSTFH
NSRTNNSQWTFQ
ESWTNSWAHYFG
ESWTNSWAMYFG
QSYTNDDVLRIS
QNMNNWTLASIM
EVMNNWTLASIM
ASISNLTLSRFM
HSWSNYWGHQHA
HRISNYAMELHS
HSLTNTQMTQLS
HSLSNIQMATLA
HRMTNAMHHFMG
HRMTNNAMDVFM
HRLTNSEQAALP
TAVTNSMMERLW
GWGNKTPSQDVH
DYTNSVSMRYLS
HQLSNKDEQTPQ
ADPFSPTNRIPL

Sequences Features:

Motif:

HSWTNSWMATFL

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning, competitive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 22508944

Target Name:

Anti-MDA-LDL monoclonal antibody LR04

Template Name:

Malondialdehyde-modified LDL (MDA-LDL)

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Control IgM was used in the negative selection, and then unbound phages were transferred to wells coated with LRO4 (positive selection). In the last biopanning round, LRO4-bound phages were competitively eluted with increasing concentrations (3-150 μg/ml) of MDA-LDL diluted in blocking buffer, followed by elution using elution buffer.

BiopanningDataSet Comments:

Dodecamer peptide sequences were aligned by the Clustal W program to obtain consensus sequences, a dodecamer linear peptide P1 (HSWTNSWMATFL).

Curator:

Curation Date:

0000-00-00

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