BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1973

Peptides:

Sequences

FLSPAQRFELFP(4)
WPSAAEAFSKSP(3)
APSAADQFMALF(2)
MPSAVHLFETMR(1)
DNSAAQYFSRMP(1)
NSAAYSFTLFPG(1)
QSAASLFNNSMN(1)
SAADRFRTGPMP(1)
SAADRFSRYHER(1)
GASAAENFLKSL(1)
DSAASHFTTQFR(1)
KSAAEQFSLTMA(1)
SAANKFTVATMP(1)
GASAVTRFQSMY(2)
SAADHWRLSALI(1)
YQSAASKWSTYY(1)
SAASKWHNALAQ(1)
SAVHQWATQFLM(1)
SAVNNWESFYGW(1)
SHTAAHHFQVML(1)
SMTAATVFWTQM(1)
YNTAATKFSLQL(1)
STAVQQFQNTLS(1)
VSTAVSRFEALT(1)
AEPAAARWQASL(1)
QPAAVRLFEMMT(1)
EPNIADFFYRSM(1)
ADQNSFISALFQ(1)
TQNMHKFFEQES(1)
AKITRTLSLPFS(2)
QKITTTFFSPLM(1)
AKLTSTFSESPH(1)
SKLTSTLTQLAS(1)
AHPITQHDTSAR(1)
SPLLDLLKLRSP(1)
SPQQQTLATFFG(1)
APIQDNTLGRWF(1)
TFPQNVPLPILP(1)
EHEQHLSLQRWF(1)
EELFTRNVLNWP(1)
SIAEKWTTYLRK(1)
KSLSRHDHIHHH(22)
KSVSSQDHIHHQ(1)
RMNSPLDVLHHR(1)
SSLHTHQTPMFM(1)

Sequences Features:

Motif:

P-S-A-[AV]-X-R-[FW]-[ES]-L-S-P, A-K-[IL]-[TQ]-X-T-L-X-L, K-S-L-S-R-X-D-X-I

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 22716192

Target Name:

Anti-MgPa polyclonal antibody

Template Name:

Adhesin P1

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In each round, the phage eluates were added into the microtiter plate coated with normal rabbit antiserum at room temperature and incubated for 1 h. The preincubation procedures were performed 5 times to remove those phages that possibly nonspecifically bind to rabbit antiserum.

BiopanningDataSet Comments:

Results of bioinformatics analysis by MIMOX demonstrated that the key consensus amino acid residues in the aligned mimotopes may be S, A, and F for cluster 1; A, K, I, T, and L for cluster 2; and K, S, L, R, D, and I for cluster 3. Three representative phages could recognize sera from M. genitalium-positive patients to varying degrees, whereas they could not recognize the sera from Mycoplasma pneumoniae-positive patients or the sera from healthy people. These findings will help to clarify the mimic epitopes of MgPa to facilitate diagnosis of the antigen and to understand the antigenic structure of MgPa.

Curator:

Curation Date:

0000-00-00

First |

Prev |

1973

| Next

| Last