BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1971

Peptides:

Sequences
TTSVLLHCIEWTILNQ

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 22683611

Target Name:

Caspase-6, CASP-6

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Linear-lib M13 phage display library

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

In each round, biotinylated zymogen caspase-6 was incubated with the amplified phage from previous round and the caspase-6-phage complex was captured by NeutrAvidin-coated plate that has been previously blocked by block buffer.

BiopanningDataSet Comments:

After four rounds of binding selection, individual phage clones were analyzed in a high-throughput phage spot ELISA using plate-immobilized zymogen caspase-6 as the target. The binding signal of the same phage particle to NeutrAvidin was detected as non-specific binding noise. Clones with phage-binding signal to target over 0.5 and signal/noise ratio > 3 were considered to be positive clones and were subjected to DNA sequence analysis.

Curator:

Curation Date:

0000-00-00

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