BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1947

Peptides:

Sequences

Affinity Values

QEINSSY
SHPRLSA
SMPNPMV
GLQQVLL
HELSVLL
YAPQRLP
TPRTLPT
APVHSSI
APPHALS
TFSNRFI
VVPTPPY
ELAPDSP

[0.65 ± 0.02]
[0.72 ± 0.05]
[0.69]
[0.79 ± 0.04]
[0.63 ± 0.04]
[0.58]
[0.51 ± 0.03]
[0.45 ± 0.02]
[0.59]
[0.40 ± 0.02]
[0.76 ± 0.06]
[0.59]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 22363498

Target Name:

Anti-LPS monoclonal antibody

Template Name:

Lipopolysaccharide, LPS

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-7 phage display library (X7)

Affinity Measurement Method:

ELISA

Affinity Measurement Description:

In phage ELISA, absorbance was measured at 490 nm. Each experiment was repeated three times. Data were reproduced from the graph and expressed as means ± SD. Phage 2Ab as negative control showed no ELISA signal.

Brief Description:

BiopanningDataSet Comments:

The data demonstrate the identification of synthetic peptides that mimic LPS by interacting with TLR-4. This LPS mimotope-TLR-4 interaction will allow for the development and use of these peptides as a new class of adjuvants, namely TLR-4 agonists.

Curator:

Curation Date:

0000-00-00

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