BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1939

Peptides:

Sequences
HWGNHSKSHPQR(20) KSLSRHDHIHHH(5)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 22265946

Target Name:

Anti-RPV-H monoclonal antibody C1

Template Name:

Hemagglutinin glycoprotein

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

BiopanningDataSet Comments:

Four independent peptide library screenings yielded 80 fourth-round phage clones that were selected by the C1 mAb. In twenty phage clones the amino acid sequence HWGNHSKSHPQR (Domain 1 (D1)) was obtained, whilst five phage clones had the amino acid sequence KSLSRHDHIHHH (D2). The alignment analysis with the RPV RBOK H protein revealed that residues within D1 aligned to amino acids 309-320 in H, whereas residues within D2 aligned to amino acids 529-540.

Curator:

Curation Date:

0000-00-00

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