BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1891

Peptides:

Sequences
RGWCRPLPKGEG(14)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 22086793

Target Name:

Sera from early-stage primary HCC patients

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Microtiter wells were respectively coated overnight with 100ml of purified IgG (100μμg/ml) from each of the control groups. The plates were blocked with 3% nonfat milk for 2 hr, and then washed five times with 0.05% Tween-20 in Tris-buffered saline (PBST). One hundred ?`l of diluted random 12-peptide phage display library with a titer of 1.5e11 pfu/ml was added to coated plates. After sequential incubation for 1 hr at room temperature with each of the control IgG-coated plates, the unbound phages were collected and added 100μl/well into the early HCC-IgG-coated plate. Another two rounds of affinity selection were carried out in the same way, except that 1:200 and 1:400 sera dilution was added to 100μl of diluted phages from the last round.

BiopanningDataSet Comments:

In the screening phase, 19 out of 20 randomly selected phage clones exhibited specific reaction with purified sera IgG from early primary HCC patients, among them 14 coming from the same phage clone with inserted peptide sequence RGWCRPLPKGEG (named HC1). The HC1 mimic peptide showed high diagnostic validity for early primary HCC, and thereby could be a candidate serum biomarker for early primary HCC.

Curator:

Curation Date:

0000-00-00

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