BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1878

Peptides:

Sequences	Affinity Values
WHWRNPDFWYLK(10) SNNLYPQRAVST(1) LETEWDSLWYAP(1)	[0.83] [0.07] [0.11]

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (common panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 22003385

Target Name:

Alanine aminotransferase 1, ALT1

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

ELISA,Quartz crystal microbalance (QCM)

Affinity Measurement Description:

In phage ELISA, ALT binding of the selected phage clones and M13 control phage incubated with varying concentrations of phage particles ranging from e5 pfu to e11 pfu. All measurements were performed in triplicate. The absorbance at 490 nm was reproduced and shown as means of three measurements when the concentration of phage particles is e11 pfu. The absorbance of M13 control phage is 0.07. Besides the apparent dissociation constant (Kd,app, nM) for the ALT5-8 clone with the sequence of WHWRNPDFWYLK is determined by further ELISA experiments. Further, the inhibition constant for the ALT5-8 peptide was found to be KI=71 ± 17 nM. A dissociation constant of Kd= 2.01 ± 0.06 mg/mL or 20.1 ± 0.6 nM was calculated for ALT binding on QCM.

Brief Description:

BiopanningDataSet Comments:

Phage particles expressing this peptide exhibited nanomolar affinity for immobilized ALT (Kd,app = 85±20 nM).

Curator:

Curation Date:

0000-00-00

First |

Prev |

1878

| Next

| Last