BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1863

Peptides:

Sequences
RGGRCLLCCLCLWWA AVAGGRSVVDARVAR RTEVPVLSFTSPLTG PFARAPVEHHDVVGL RVPPRYHAKISPMVK

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning, in vivo)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 20692225

Target Name:

Human DA-MB-435eb.1 tumor xenografts

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

f3-15mer phage display library (X15)

Affinity Measurement Method:

Affinity Measurement Description:

Brief Description:

Before positive selections, the library was subjected to negative selection by passage through non-tumor-bearing mice.

BiopanningDataSet Comments:

Following in vivo selection from the f3-15mer phage display library in mice harboring tumor xenografts, two phage clones (RGGRCLLCCLCLWWA and AVAGGRSVVDARVAR) predominated in final outputs. These clones had no discernible affinity for the tumor cell line in vivo or in vitro but had unusually high infectivities, prompting further investigation.

Curator:

Curation Date:

0000-00-00

First |

Prev |

1863

| Next

| Last