BDB -BiopanningDataSet Details

Biopanning Data

BiopanningDataSet ID: 1853

Peptides:

Sequences
DRYSEIRTSSTL(6) YSGLQDSSLRLR(4) DYPSANKWPRYV(1)

Sequences Features:

Motif:

Unique Sequence Number:

Experimental Method:

Phage display (subtractive panning)

Sequencing Platform:

Sequencing Method:

Sanger sequencing

Rounds of Panning:

Reference:

PMID: 21930161

Target Name:

The tegument of schistosoma japonicum schistosomula

Template Name:

Not determined.

Structure of Target-Template Complex:

Not determined.

Structure of Target-Peptide Complex:

Not determined.

Library Name:

Ph.D.-12 phage display library (X12)

Affinity Measurement Method:

Western blot

Affinity Measurement Description:

To confirm the binding ability of positive phages, the MppZL1 (DYPSANKWPRYV), MppZL4 (YSGLQDSSLRLR), MppZL6 (DRYSEIRTSSTL) and negative control M13KE phage were tested for recovery rate. Significantly higher binding was observed by MppZL4 to schistosomula than by MppZL6 or MppZL1 or control M13KE phage. The results of Western blot indicated that the peptide of the MppZL4 exhibited strong affinity for the surface membrane or tegument of schistosomula.

Brief Description:

Cercariae was directly used for construction of a reverse adsorption peptide library.

BiopanningDataSet Comments:

Of the three, M13 phage peptide ZL4 (MppZL4, YSGLQDSSLRLR, 1.4 kDa, pI 8.8) bound to the tegument of mechanically transformed schistosomula and to other developmental stages of S. japonicum from the mammalian host. By contrast, MppZL4 did not bind to the surface of cercariae.

Curator:

Curation Date:

0000-00-00

First |

Prev |

1853

| Next

| Last